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Germination Rate and Moisture Content of Eelgrass, Zostera Marina Upon Desiccation

잘피, 거머리말 종자의 건조에 따른 발아율과 함수율

JUNG-IM PARK1
,
JONG-HYEOB KIM1
,
JONG-RYOL KIM2
박 정임1
,
김 종엽1
,
김 종열2
1Marine-Eco Technology Institute
2Korea Fisheries Resources Agency
1해양생태기술연구소
2한국수산자원관리공단 생태복원실
August 2017. pp. 57-68
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Abstract

거머리말은 해양 현화 식물로 일생의 대부분을 해수 내에서 생활하며, 공기에 노출시 건조 스트레스를 받게 된다. 지금까지 거머리 말 성체에서 나타나는 건조 스트레스는 다수 알려진데 비해, 거머리말 종자의 건조 스트레스에 대한 정보는 부족한 편이다. 이 연구 는 거머리말 종자의 건조 스트레스를 알아보기 위해 공기 중 노출로 인한 종자의 발아율과 함수율의 변화를 실험하였다. 상대습도 50%, 20°C의 공기에 1시간 노출된 거머리말 종자의 발아율은 현저히 감소되었으며, 노출시간이 길어질수록 종자의 발아율은 지속 적으로 감소하였고, 11시간 이상 노출된 종자는 전혀 발아하지 않았다. 거머리말 종자의 공기 노출시간이 길어질수록 발아율과 함 수율은 감소하는 음의 상관관계가 나타났다. 또한, 30°C의 공기에 노출된 거머리말 종자는 10°C와 20°C의 공기에 노출된 거머리말 종자보다 현저히 낮은 발아율을 나타내었다. 따라서 거머리말 성체가 공기 중 노출 시 건조 스트레스를 겪는 것처럼 거머리말 종자도 건조에 의한 발아율의 감소가 확인되었으며, 이것은 종자를 활용한 거머리말 서식지 복원 사업에 유용한 정보를 제공하고자 한다.

Eelgrass, Zostera marina is a marine flowering plant that grows in submerged habitats for most of its lifetime, and experiences desiccation stress when exposed to air. Although the desiccation stress observed in adult eelgrass has been frequently studied, there has been little research on desiccation stress in eelgrass seeds. To survey desiccation stress in eelgrass seeds, we studied the germination rate and moisture content upon desiccation caused by exposure to air. The germination rate of the eelgrass seeds exposed to air for 1 hour at 20°C and 50% relative humidity decreased markedly, and it decreased consistently as the exposure time increased. Eelgrass seeds exposed to air for longer than 11 hours did not germinate. As the exposure time of eelgrass seeds to air increased, the germination rate and moisture content decreased, showing a negative correlation. In addition, eelgrass seeds exposed to air at 30°C showed significantly lower germination rates than those exposed to air at 10°C or 20°C. Our results revealed that desiccation causes a decrease in the germination of eelgrass seeds, which will provide useful information for eelgrass habitat restoration using seeds.

Keywords
Eelgrass
Zostera marina
Seed
Desiccation stress
Germination

References
1
Chin, H.F., 1995. Storage of recalcitrant seeds. In: Seed quality basic mechanisms and agriculture implications, edited by Basra, A.S., The Haworth Press Inc, New York, pp. 209-222.
2
Churchill, A.C., 1983. Field studies on seed germination and seedling development in Zostera marina L. Aquat. Bot., 16: 21-29.
3
den Hartog, C., 1970. The seagrass of the world. North-holland Publishing Company, Amsterdam, Netherlands, 275 pp.
4
Duarte, C.M., 2000. Marine biodiversity and ecosystem services: an elusive link. J. Exp. Mar. Biol. Ecol. 250: 117-131.
5
Fonseca, M.S., W.J. Kenworthy and G.W. Thayer, 1998. Guidelines for the conservation and restoration of seagrasses in the United States and adjacent waters. NOAA Coastal Ocean Program/Decision Analysis Series NO. 12. NOAA Coastal Ocean Office, Silver Spring, MD, 222 pp.
6
Hay, F., R. Probert, J. Marro and M. Dawson, 2000. Towards the ex situ conservation of aquatic angiosperms: a review of seed storage behaviour, edited by Black, M., K.J. Bradford and J. Vázquez-Ramos, CABI Publishing, New York, 161-178 pp.
7
Hovel, K.A., M.S. Fonseca, D.L. Meyer, W.J. Kenworthy and P.G. Whitfield, 2002. Effects of seagrass landscape structure, structural complexity and hydrodynamic regime on macrofaunal densities in North Carolina seagrass beds. Mar. Ecol. Prog. Ser., 243: 11-24.
8
Kauth, P.J. and P.D. Biber, 2014. Moisture content, temperature, and relative humidity influence seed storage and subsequent survival and germination of Vallisneria americana seeds. Aquat. Bot., 120: 297-303.
9
Kim, J.B., W.-C. Lee, K.-S. Lee and J.-I. Park, 2013. Growth dynamics of eelgrass, Zostera marina, in the intertidal zone of Seomjin Estuary, Korea. Ocean Sci. J., 48: 1-12.
10
Lee, K.-S. and S.Y. Lee, 2003. The seagrasses of the Republic of Korea. In: World Atlas of Seagrasses: Present Status and Future Conservation, edited by Green, E.P., F.T. Short and M.D. Spalding, University of California Press, Berkeley, 193-198 pp.
11
Leuschner, C., S. Landwehr and U. Mehling, 1998. Limitation of carbon assimilation of intertidal Zostera noltii and Z. marina by desiccation at low tide. Aquat. Bot. 62: 171-176.
12
Orth, R.J., M.L. Luckenbach, S.R. Marion, K.A. Moore and D.J. Wilcox, 2006. Seagrass recovery in the Delmarva Coastal Bays, USA. Aquat. Bot., 84: 26-36.
13
Pan, J., H. Han, X. Jiang, W. Zhang, N. Zhao, S. Song, X. Li, and X. Li, 2012. Desiccation, moisture content and germination of Zostera marina L. seed. Restor. Ecol., 20: 311-314.
14
Pan, J., J. Xin, L. Xiaojie, C. Yizhou, Z. Zhuangzhi, L. Zhiling, Z. Weili, H. Houwei, L. Shiju and Y. Guanpin, 2011. Influence of temperature and salinity on germination of eelgrass (Zostera marina L.) seeds. J. Ocean Univ. China, 10: 147-152.
15
Park, J.-I. and K.-S. Lee, 2007. Site-specific success of three transplanting methods and the effect of planting time on the establishment of Zostera marina transplants. Mar. Pollut. Bul., 54: 1238-1248.
16
Park, J.-I., K.-S. Lee and M.H. Son, 2012. Germination rate of Zostera marina and Phyllospadix japonicus related to environmental factors. Korean J. Environ. Biol., 30: 280-285.
17
Shafer, D.J. and P. Bergstrom, 2010. An introduction to a Special Issue on Large-scale submerged aquatic vegetation restoration research in the Chesapeake Bay: 2003-2008. Restor. Ecol., 18: 481-489.
18
Shafer, D.J., Sherman, T.D., and S. Wyllie-Echeverria, 2007. Do desiccation tolerances control the vertical distribution of intertidal seagrasses? Aquat. Bot., 87: 161-166.
19
Tanaka, Y. and M. Nakaoka, 2004. Emergence stress and morphological constraints affect the species distribution and growth of subtropical intertidal seagrasses. Mar. Ecol. Prog. Ser., 284: 117-131.
20
Tanner, C.E. and T. Parham, 2010. Growing Zostera marina (eelgrass) from seeds in land-based culture systems for use in restoration projects. Restor. Ecol., 18: 527-537.
21
Thomas, F.I.M. and C.D. Cornelisen, 2003. Ammonium uptake by seagrass communities: effects of oscillatory versus unidirectional flow. Mar. Ecol. Prog. Ser., 247: 51-57.
Information
  • Publisher :The Korean Society of Oceanography
  • Publisher(Ko) :한국해양학회
  • Journal Title :The Sea Journal of the Korean Society of Oceanography
  • Journal Title(Ko) :한국해양학회지 바다
  • Volume : 22
  • No :3
  • Pages :57-68
  • Received Date : 2017-07-10
  • Revised Date : 2017-08-10
  • Accepted Date : 2017-08-11
  • DOI :https://doi.org/10.7850/jkso.2017.22.3.069
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