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2020 Vol.25, Issue 2 Preview Page

Article

Studies on the Optimal Conditions of Feeding and Light Supply for the Long-Term Cultivation of Meiofauna in the Laboratory

중형저서동물의 실험실 내 장기 배양을 위한 먹이 및 광원의 최적 조건 연구

AYOUNG SHIN1
,
DONGSUNG KIM2*
,
TEAWOOK KANG3
,
JE HYEOK OH1
신 아영1
,
김 동성2*
,
강 태욱3
,
오 제혁1
1Research Specialist, Marine Ecosystem Research Center, KIOST
2Senior Researcher, Marine Ecosystem Research Center, KIOST
3Senior Researcher, Marine Research Center, Korea National Park service
1한국해양과학기술원 해양생태연구센터 무기계약직기술원
2한국해양과학기술원 해양생태연구센터 책임연구원
3국립공원공단 국립공원연구원 해양연구센터 책임연구원
* Corresponding Author
31 May 2020. pp. 26-41
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Abstract

본 연구는 중형저서동물의 생리·생태적 연구를 위한 생물을 배양하기 위해 다양한 환경 조건을 설정하여 중형저서동물이 실험실 내에서 가장 이상적으로 배양되는 조건을 파악하고자 하였다. 배양에 사용된 퇴적물과 해수는 서해안 만리포 조간대에서 채집하였다. 채집된 중형저서동물은 항온·항습 기능이 있는 챔버 내에 환경 조건을 조절할 수 있는 수조를 제작하여, 수조 내에 채집된 해수와 퇴적물과 함께 배양 실험을 수행하였다. 실험 1은 시료 채집 지역과 유사한 환경을 조성하여 실험을 수행하였다. 실험 2는 실험 1과 같은 조건에 먹이생물 공급 유무의 차이를 확인하였다. 실험 3에서는 빛과 먹이생물 공급의 유무의 차이에 따른 중형저서동물 군집의 변화를 확인하였다. 배양 실험 결과, 중형저서동물 군집은 먹이공급을 하지 않은 수조에 비하여 먹이 공급을 한 수조, 빛이 없는 조건보다 12시간 주기의 빛이 있는 조건에서 서식밀도와 출현분류군수가 상대적으로 높게 나타났다. 또한 실험실 내에서 배양된 중형저서동물의 서식밀도는 자연 상태에 비하여 상대적으로 높은 값을 나타냈다.

In order to culture a life for the physiological and ecological research of the meiofauna, this study aimed to identify the most ideal condition in which the meiofauna can be cultured within a laboratory by setting various environmental conditions. The sediment deposits and seawater were collected from the intertidal zone in Mallipo of the west coast. A aquarium in which the internal environment can be controlled by constantly maintaining the temperature and humidity was fabricated and the culture experiments of the collected meiofauna were conducted together with the sea water and sediment deposits collected. The experiment 1 was conducted after establishing the similar environment as the collecting location. Under the same condition as the experiment 1, the experiment 2 verified a difference between when live foods were supplied and were not. In the experiment 3, the changes in the meiofauna colony were checked according to with or without light and live foods. In the results of culturing experiments, the habitat density and the number of appeared classification groups of the meiofauna colony were relatively higher both in the water tank with supplying the live foods and under the condition of having light in 12-hour cycle than those in the aquarium without live foods and under no light condition. In addition, the habitat density of meiofauna cultured within a laboratory exhibited relatively higher value than that under the natural state.

Keywords
Meiofauna
Meiofuana culture
Feeding condition
Light condition
Cultivation

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Information
  • Publisher :The Korean Society of Oceanography
  • Publisher(Ko) :한국해양학회
  • Journal Title :The Sea Journal of the Korean Society of Oceanography
  • Journal Title(Ko) :한국해양학회지 바다
  • Volume : 25
  • No :2
  • Pages :26-41
  • Received Date : 2020-01-22
  • Revised Date : 2020-04-28
  • Accepted Date : 2020-05-13
  • DOI :https://doi.org/10.7850/jkso.2020.25.2.026
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